Expanding the Toolbox of R-Selective Amine Transaminases by Identification and Characterization of New Members

Amine transaminases (ATAs) are used to synthesize enantiomerically pure amines, which are building blocks for pharmaceuticals and agrochemicals. R-selective ATAs belong to the fold type IV PLP-dependent enzymes, and different sequence-, structure- and substrate scope-based features have been identified in the past decade. However, our knowledge is still restricted due to the limited number of characterized (R)-ATAs, with additional bias towards fungal origin. We aimed to expand the toolbox of (R)-ATAs and contribute to the understanding of this enzyme subfamily. We identified and characterized four new (R)-ATAs. The ATA from Exophiala sideris contains a motif characteristic for d -ATAs, which was previously believed to be a disqualifying factor for (R)-ATA activity. The crystal structure of the ATA from Shinella is the first from a Gram-negative bacterium. The ATAs from Pseudonocardia acaciae and Tetrasphaera japonica are the first characterized (R)-ATAs with a shortened/missing N-terminal helix. The active-site charges vary significantly between the new and known ATAs, correlating with their diverging substrate scope.     

Crisis communication,anticipated food scarcity,and food preferences: Preregistered evidence of the insurance hypothesis

Whereas large-scale consumption of energy-dense foods contributes to climate change, we investigated whether exposure to climate change-induced food scarcity affects preferences toward these foods. Humans’ current psychological mechanisms have developed in their ancestral evolutionary past to respond to immediate threats and opportunities. Consequently, these mechanisms may not distinguish between cues to actual food scarcity and cues to food scarcity distant in time and space. Drawing on the insurance hypothesis, which postulates that humans should respond to environmental cues to food scarcity through increased energy consumption, we predicted that exposing participants to climate change-induced food scarcity content increases their preferences toward energy-dense foods, with this effect being particularly pronounced in women. Three experiments—including one preregistered laboratory study—confirm this notion. Our findings jointly demonstrate that receiving information about food shortages distant in time and space can influence current food preferences.     

鄂尔多斯盆地延长组致密砂岩储层微观孔喉特征差异及其成因

为分析不同沉积与成岩作用改造下致密砂岩储层的孔喉类型及其参数差异，利用物性、铸体薄片、扫描电镜、恒速压汞、图像粒度与X衍射实验结果，对比了鄂尔多斯盆地合水地区延长组长8储层和薛岔地区延长组长6储层的孔喉类型，定量评价了孔喉参数，揭示了形成原因。结果表明，合水地区长8储层孔隙更为发育，粒间孔、溶蚀孔、晶间孔和微孔、弱压实成因缩颈状喉道和溶蚀成因、粘土胶结成因管束状喉道含量高；薛岔地区长6储层的孔隙类型少且发育程度较差，压实成因片状、弯片状喉道含量高。两个区块的孔隙参数差异小，喉道参数差异大，前者大喉道更为发育，喉道差异大，孔喉比大，对渗透率起贡献的喉道分布范围更宽。相同渗透率条件下，前者的平均喉道半径、主流喉道半径和孔喉比大于后者。沉积水动力条件、粒度、碎屑组分、填隙物、成岩作用类型及其改造程度是两个研究区孔隙发育程度不同、喉道类型差异和孔喉参数表现出不同特征的主要原因。     

Yoghurt‐type beverage with partial substitution of milk by a chickpea extract: Effect on physicochemical and flow properties

The objective of this study was to determine the evolution of physicochemical and flow properties of a yoghurt‐type beverage partially replaced with a chickpea extract over a period of 22 days. From two chickpea extracts, Blanco Noroeste was selected for its higher protein content. Six yoghurt‐type formulations were prepared, stored at 4 °C and analysed on days 1, 8, 15 and 22. pH was 4.24–4.75, while brightness was 81.7–94.5. Flow properties of the yoghurt‐type exhibited a non‐Newtonian behaviour. From three formulations that underwent sensory evaluation, the 30:70 mix obtained the highest acceptation. This yoghurt‐type beverage exhibited characteristics similar to natural yoghurt.     

The cumulative power of incremental innovation and the role of project sequence management

In innovation and project management studies incremental development projects are perceived as theoreticlly and organisationally uninteresting. By means of a longitudinal study of product improvement projects at an automobile firm, this paper challenges such views and shows how the cumulative impact of the studied sequence resulted in a competitive repositioning of the company's product portfolio during a financially difficult period. Project managers achieved this by transcending the separation between exploration and exploitation projects; they not only adhered to time, cost and quality goals but also tried out new ways of testing and experimenting with controversial technical ideas. The paper analyzes the intensive inter project learning that generated these ambidextrous capabilities and emphasizes that practices at the project-level need to be buttressed by expanded management learning and capability development also at the sequence level.     

水蒸气对煤焦油模化物裂解行为及析碳的影响

采用机械化学法制备Ni-Ce/Al₂O₃催化剂，在固定床反应器中考察了水蒸气气氛对煤焦油模型化合物甲苯＋芘催化裂解行为的影响。根据产物生成规律提出了芘向萘转化的裂解机理，并以D₂O对其进行了验证。通过XRD、TG-DTG和Raman等表征了析碳的类型与结构特征。结果表明：相较于纯氮气气氛，水的加入可明显提升重质组分芘的裂解率，且随水碳比（S/C）增加呈先增加后降低的趋势，在S/C=0.15时达到最大值98.93%，比S/C=0时增加32.09%。析碳率随S/C比增加一直呈下降趋势，由S/C=0时的10.04%降至S/C=0.26时的5.39%。析碳分析结果表明，S/C=0时，生成的积炭类型主要是β型碳及γ型碳，水蒸气存在时，活性较高的α型碳含量增加，说明水蒸气的持续消碳作用抑制了C_α向C_β与C_γ方向转化。     

Effect of an abrupt change in pulling rate on microstructures of directionally solidified Al2O3-Er3Al5O12 eutectic and off-eutectic composite ceramics

Directionally solidified microstructures of Al₂O₃-Er₃Al₅O₁₂ eutectic and off-eutectic in situ composite ceramics were explored under abrupt-change pulling rate conditions. Corresponding temperature distributions and interface locations were studied. In eutectic composition, fluctuation of eutectic spacing occurred when the pulling rate increased abruptly. A gradually increase or abrupt increase in eutectic spacing was observed when the pulling rate decreased abruptly. In hypoeutectic and hypereutectic compositions, formation of the primary phases were suppressed when the pulling rate increased abruptly from 10?µm/s to 100?µm/s, while primary phases precipitated when the pulling rate decreased abruptly from 100?µm/s to 10?µm/s. The interface altitude decreased after the pulling rate increased abruptly, but increased after the pulling rate decreased abruptly. The liquid composition restriction (around the eutectic composition) at the eutectic interface plays an important role in the suppression of the primary dendrite and coupled eutectic oxides can be obtained in off-eutectic compositions even under higher solidification rate conditions.     

WSNs中规避攻击者的源位置隐私保护路由协议

为了保证无线传感器网络（Wireless Sensor Networks，WSNs）中的源节点位置隐私安全，同时实现安全性能和网络能耗的均衡，提出了WSNs中规避攻击者的源节点位置隐私路由协议，该协议假设节点具有检测攻击者的能力，通过发出危险警告消息使路由路径上的节点采取路由改变策略，使攻击者无法回溯到源节点，延长了源节点保持位置隐私的安全时间。理论分析和仿真实验表明，该协议在消耗较少的通信开销的情况下保证了源节点位置的高度隐私。     

基于时间延迟的多类型维修与经济生产批量联合优化研究

针对目前未能较好的综合考虑多维修类型与经济生产批量联合优化的问题，首先考虑多类型维修关系，基于时间延迟理论求出故障和缺陷次数的表达式；其次，在此基础上，综合考虑生产费用和维修费用的基础上，构建了多类型维修和经济生产批量联合优化模型，以单位时间内总费用最小为优化目标，获得最优检查间隔期和经济生产批量；最后，通过算例分析验证了模型的有效性，说明了第一类缺陷检查次数的多少对费用和经济生产批量影响不大。     

结合MNF变换和Canny算子的遥感影像变化检测

针对大面积区域的多时相遥感影像变化检测的需求，提出了一种基于最小噪声分离（MNF）的Canny边缘检测提取影像变化信息的检测方法。对多时相影像采用多种变换组合成具有多维波段信息的影像，采用最小噪声分离法分离噪声并得到单波段差异图，通过Canny边缘检测法计算梯度幅值，采用高低双阈值法细化边缘，从而提取差异图变化边缘，有效突出了变化信息。以1995年和2003年加扎勒河的两期遥感影像为例，利用两时相影像进行土地覆被变化检测。实验结果表明，该方法适用于监测大面积区域内地物的突变情况。在数据基础上进行最小噪声分离可以有效解决传统Canny边缘检测提取边缘时造成的伪边缘现象，同时采用高低双阈值法有效去除伪边缘点，从而获得更加精确、直观的变化检测效果，在自然地理变化监测、地理国情灾害监测等有很好的应用价值。